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The Eno River, named for the Eno Indians who once lived along its banks, is the initial tributary of the Neuse River in North Carolina, USA.
The Eno rises in Orange County. The river's watershed occupies most of Orange and Durham counties. The Eno converges with the Flat and Little Rivers to form the Neuse at Falls Lake, which straddles Durham and Wake counties.
The Eno is notable for its beauty and water quality, which has been preserved through aggressive citizen efforts. Though barely more than forty miles from its source to its convergence at the Neuse, the Eno features significant stretches of natural preservation. Through the combined efforts of the North Carolina State Parks System, local government, and private non-profit preservation groups, over 5,600 acres (23 km2) of land have been protected in the Eno Basin, including Occoneechee Mountain State Natural Area, Eno River State Park, West Point on the Eno (a Durham City Park), and Penny's Bend State Nature Preserve (managed by the North Carolina Botanical Garden).